Pengaruh Kecepatan dan Waktu Sentrifugasi terhadap Nilai Hematokrit Mahasiswa AAK Manggala Yogyakarta

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Jeniwati Tamo Inya
Akademi Analis Kesehatan Manggala Yogyakarta
Pratiwi Ratih Halimatus Sya'diah
Akademi Analis Kesehatan Manggala Yogyakarta
Ardina Nugrahani
Akademi Analis Kesehatan Manggala Yogyakarta

A hematocrit test is an examination that aims to diagnose a disease related to the number of red blood cells (erythrocytes). In the laboratory, there are often errors in hematocrit examinations, namely with the inaccuracy of the centrifuge time, with the large number of patients it is one of the obstacles for laboratory staff in conducting examinations, so that the centrifuge is turned off faster and sometimes also late. The purpose of this study is to determine the effect of centrifugation speed and time on hematocrit levels of students in the fourth semester of AAK Manggala Yogyakarta in 2023. This research method is descriptive analytical. The sample of this study is 39 samples. This study uses the microhematocrit method at 15,000 rpm, 12,000 rpm, 10,000 rpm, 7,000 rpm for 5 and 10 minutes with data analysis using the Kruskal Wallis test. The results showed that the average hematocrit value in men was 40% and women was 40%. The results of the statistical test show the significance value 15,000 rpm for 5 and 10 minutes is p=0.631 (p>0.05), 12,000 rpm during 5 and 10 minutes is p= 0.255 (p>0.05), 10,000 rpm for 5 and 10 minutes is p= 0.496 (p>0.05) at 7,000 rpm for 5 and 10 minutes is p=0.316 (p>0.05). The conclusion of this study is that there is no effect of speed and time of snetrifugation on hematocrit values. (p> 0.05).


Keywords: centrifugation speed, centrifugation time
Bakta, I. M. (2017). Pendekatan Diagnosis dan Terapi pada Penderita Anemia. Bali Health Journal, 1(1), 36–48.
Chairani, C., Susanto, V., Monitari, S., & Marisa, M. (2022). Nilai Hematokrit pada Pasien Hemodialisa dengan Metode Mikrohematokrit dan Automatik. Jurnal Kesehatan Perintis, 9(2), 89–93.
Elly Ernawati, E. (2019). Gambaran Hasil Pemeriksaan Hematokrit Secara Manual dan Automatik pada Pasien Rawat Inap di RSUD Lubuk Sikaping. Stikes Perintis Padang.
Hidayat, F., Yogie, G. S., Firmansyah, Y., Santoso, A. H., Kurniawan, J., Amimah, R. M. I., Gaofman, B. A., & Syachputri, R. N. (2023). Gambaran Kadar Hemoglobin dan Hematokrit pada Wanita Usia Produktif. MAHESA: Malahayati Health Student Journal, 3(11), 3629–3636.
Jiwintarum, Y., Srigede, L., & Asyhaer, R. K. (2020). Hematocrite Values With High Measurement of Eritrosit After Centrifugation on Serum Making. Jurnal Analis Medika Biosains (JAMBS), 7(2), 112–121.
Maharani, E. A., & Eka, A. M. (2020). Hematologi Teknologi Laboratorium Medik. Jakarta: EGC.
Majid, N., Manik, S. E., & Pramitaningrum, I. K. (2023). Pengaruh Variasi Waktu Sentrifugasi Nilai Hematokrit Menggunakan Metode Mikrohematokrit di Universitas Binawan. Jurnal Sainhealth Vol, 7(2).
Meilanie, A. D. R. (2019). Different of Hematocrit Value Microhematocrit Methods and Automatic Methods in Dengue Hemorrhagic Patients With Hemoconcentration. Journal of Vocational Health Studies, 3(2), 67–71.
Melinia, P. S. (2021). Pengaruh Kecepatan dan Waktu Sentrifugasi terhadap Kadar Hematokrit Mahasiswa Prodi DIII TLM Poltekkes Kemenkes Palembang Tahun 2021.
Nugraha, G. (2017). Panduan Pemeriksaan Laboratorium Hematologi Dasar Edisi 2. Jakarta: Trans Info Media.
Nugrahani, W. (2018). Perbedaan Nilai Hematokrit Darah Edta Metode Autoanalyzer dengan Mikrokapiler pada Tersangka Demam Berdarah Dengue. Universitas Muhammadiyah Semarang.
Nuradi, N., & Jangga, J. (2020). Hubungan Kadar Hemoglobin dan Nilai Hematokrit pada Perokok Aktif. Jurnal Media Analis Kesehatan, 11(2), 150–158.
Nuraeni, M. (2020). Perbandingan Nilai Hematokrit Darah Vena Metode Automatik dan Darah Kapiler Metode Mikro Hematokrit. Perbandingan Nilai Hematokrit Darah Vena Metode Automatik Dan Darah Kapiler Metode Mikro Hematokrit, 3(2), 295–300.
Oktaviani, N. A. (2018). Perbedaan Waktu dan Kecepatan Centrifuge Terhadap Nilai Hematokrit Metode Mikrohematokrit. Universitas Muhammadiyah Semarang.
Pangestu, G. (2019). Gambaran Lama Dan Kecepatan Sentrifugasi terhadap Kadar Hematokrit pada Mahasiswa Semester VI D-III Analis Kesehatan Stikes Icme Jombang. Stikes Insan Cendekia Medika Jombang.
Rosidah, R., & Wibowo, C. (2018). Perbedaan Antara Pemeriksaan Antikoagulan EDTA dan Heparin terhadap Nilai Hematokrit (HCT). Jurnal Sains, 8(16).
Rosita, L., Pramana, A. A. C., & Arfira, F. R. (2019). Hematologi Dasar. Universitas Islam Indonesia.
Saadah, S. (2018). Sistem Peredaran Darah Manusia. 8 Februari, 1–58.
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